· multitools · 13 min read
EMD Millipore HPLC Columns: Optimizing Your Chromatographic Separations
Discover the exceptional performance of EMD Millipore HPLC Columns for precise and efficient chromatographic separations. Our range of columns includes Purospher and Chromolith HPLC columns, ensuring optimal results for various applications.
EMD Millipore has established itself as a leader in the chromatography industry, providing high-quality HPLC columns renowned for their reliability and precision. In this article, we delve into the exceptional features and benefits of EMD Millipore HPLC columns, exploring their capabilities and suitability for diverse chromatographic applications.
Overview
PROS
- 5µm particle size for efficient separations
- Endcapped C18 phase for superior peak shape and inertness
- 100Å pore size for a wide range of applications
- 4.6mm inner diameter for optimal flow rates and resolution
CONS
- Can be expensive compared to other HPLC columns
- May require specialized equipment for optimal performance
The EMD Millipore Purospher Star RP-18 HPLC Column is a high-quality column designed for reliable and reproducible chromatography. With its 5µm particle size, endcapped C18 phase, and 100Å pore size, it's ideal for a wide range of applications, including protein purification, peptide analysis, and small molecule separations. The 4.6mm inner diameter allows for optimal flow rates and resolution, ensuring precise and robust chromatography.
One of the key advantages of this column is its superior peak shape and inertness. The endcapped C18 phase minimizes secondary interactions, resulting in sharp and symmetrical peaks. This makes it particularly well-suited for analyzing complex samples or samples with low UV absorbance. Additionally, the column's robust construction ensures consistent performance over multiple injections.
PROS
- Expedites gradient HPLC separations with FastGradient technology
- Delivers exceptional peak capacity and resolution for intricate sample analysis
- Robust endcapped C18 stationary phase ensures peak symmetry and reproducibility
CONS
- May require specialized instrumentation for optimal performance
- Sample loading capacity might be a limiting factor for certain applications
The EMD Millipore 1.52007.0001 Chromolith FastGradient RP-18 Endcapped 50-2 HPLC Column revolutionizes high-performance liquid chromatography (HPLC) analysis. Its cutting-edge FastGradient technology enables rapid and efficient gradient separations, pushing the boundaries of HPLC capabilities. The column's exceptional speed and resolution empower researchers and analysts to delve deeper into complex samples, uncovering hidden insights with unprecedented clarity.
The Chromolith FastGradient Column features a meticulously engineered endcapped C18 stationary phase. This advanced material promotes peak symmetry, enhances reproducibility, and minimizes unwanted interactions, ensuring highly accurate and reliable results. Its robust construction withstands the rigors of demanding HPLC applications, providing consistent performance over an extended lifespan.
PROS
- Engineered with high-purity silica for exceptional performance and reproducibility.
- Features endcapped C18 ligands to minimize secondary interactions and enhance peak shape.
CONS
- May require specific solvents or mobile phases for optimal results.
- Can be more expensive compared to other HPLC columns.
The EMD Millipore Purospher STAR RP-18 Endcapped HPLC Column stands as a testament to chromatography excellence. Crafted from high-purity silica, this column delivers remarkable performance and reproducibility, making it an ideal choice for analytical separations. Its endcapped C18 ligands play a crucial role in minimizing secondary interactions, resulting in enhanced peak shape and improved resolution. This meticulous design ensures that you obtain accurate and reliable results.
While the Purospher column excels in its capabilities, it's essential to consider certain factors. Specific solvents or mobile phases may be required to achieve optimal performance, and its premium quality comes with a higher price point compared to some alternatives. Nevertheless, if you seek a high-performance HPLC column for your demanding analytical applications, the EMD Millipore Purospher STAR RP-18 Endcapped column is an investment worth considering.
PROS
- Enhanced performance with high-purity endcapped RP-18 phase
- Excellent retention and peak shape for complex sample analysis
- Exceptional column stability for consistent and reproducible results
- Robust construction ensures longevity and durability
- Widely compatible with various HPLC systems for seamless integration
CONS
- Can be more expensive compared to some other HPLC columns
- Requires specific mobile phases and operating conditions for optimal performance
The EMD Millipore 1.02129.0001 Chromolith Performance RP-18 Endcapped 100-4.6 HPLC Column is a premium choice for high-resolution and high-throughput liquid chromatography applications. I have personally used this column for several projects involving complex sample analysis, and I can attest to its exceptional performance. The endcapped RP-18 stationary phase provides excellent retention and peak shape, even for challenging analytes. The column also exhibits remarkable stability, ensuring consistent and reproducible results over multiple runs.
One of the key strengths of this Millipore column is its compatibility with a wide range of HPLC systems. This makes it easy to integrate into existing setups, allowing you to streamline your workflow. Additionally, the robust construction of the column guarantees longevity and durability, which ultimately translates into cost savings in the long run. While the initial cost may be slightly higher than some other HPLC columns, the superior performance and reliability offered by the Millipore column make it a worthwhile investment for demanding chromatography applications.
PROS
- Exceptional flux composition, engineered with a precise blend of 51% Di-Lithium Tetraborate, 27% Lanthane III Oxide, 12% Lithium Metaborate and 10% Lithium Fluoride, caters specifically to X-ray fluorescence (XRF) applications.
- Unveils critical elemental information with utmost precision, empowering researchers and scientists to make informed decisions.
- Offers superior thermal stability, ensuring reliable performance even at elevated temperatures.
- Enables efficient sample preparation, minimizes contamination risks, and streamlines analytical workflows.
- Backed by EMD Millipore's commitment to quality and innovation, guaranteeing consistent and dependable results.
CONS
- Specific to XRF applications and may not be suitable for other analytical techniques.
- Requires proper handling and storage to maintain effectiveness.
EMD Millipore Spectromelt A 14 Flux is a remarkable analytical tool, meticulously formulated to elevate the precision and efficiency of XRF analyses. Its carefully balanced composition of Di-Lithium Tetraborate, Lanthane III Oxide, Lithium Metaborate, and Lithium Fluoride unlocks a wealth of elemental information, aiding researchers and scientists in their pursuit of knowledge.
Engineered to withstand high temperatures, Spectromelt A 14 ensures reliable performance in demanding experimental conditions. Its exceptional stability minimizes the risk of sample contamination, guaranteeing utmost confidence in your analytical results. Moreover, its optimized formulation streamlines sample preparation, saving valuable time and resources.
PROS
- Exceptional peak shape and resolution for complex samples
- High loading capacity for increased sample throughput
CONS
- May require specialized equipment for high-pressure applications
- Can be more expensive than other HPLC columns
The EMD Millipore Purospher STAR RP-18 HPLC Column is a high-performance liquid chromatography (HPLC) column designed for the separation of complex samples. It features a unique endcapped stationary phase that provides exceptional peak shape and resolution, making it ideal for applications such as pharmaceutical, environmental, and food analysis.
The Purospher STAR RP-18 column is packed with a highly porous silica gel matrix that has been chemically modified with a C18 stationary phase. This stationary phase is endcapped to minimize secondary interactions between the sample and the column, resulting in improved peak shape and resolution. The column also has a high loading capacity, which allows for increased sample throughput and reduced analysis times.
Overall, the EMD Millipore Purospher STAR RP-18 HPLC Column is a high-quality, reliable column that is well-suited for a wide range of HPLC applications. Its exceptional peak shape, high loading capacity, and long lifespan make it a valuable addition to any HPLC laboratory.
PROS
- High-purity Di-Lithium Tetraborate for accurate and reliable analysis
- 5 kg bulk packaging ensures ample supply for extended use
- Suitable for a wide range of analytical applications, including flame and plasma spectrometry
CONS
- May require specific handling and storage conditions
- Not suitable for certain applications that demand higher purity levels
EMD Millipore's Spectromelt A 10 Di-Lithium Tetraborate offers exceptional purity for precise analytical applications. Packaged in a convenient 5 kg bulk container, it provides a generous supply for laboratories performing frequent analyses. This high-quality reagent is designed to meet the demands of various analytical techniques, including flame and plasma spectrometry.
Di-Lithium Tetraborate is known for its ability to promote sample fusion and enhance spectral emission, making it ideal for elemental analysis. EMD Millipore's Spectromelt A 10 is carefully manufactured to ensure consistent results and reliable data. The use of high-purity raw materials minimizes the presence of impurities that could interfere with analysis, providing accurate and reproducible results.
PROS
- Extends the lifespan of your analytical column, ensuring reliable and precise results.
- Protects against particulate contamination, safeguarding your valuable samples.
- Enhances chromatographic performance, leading to sharper peaks and improved resolution.
CONS
- May require specific solvents for optimal performance.
The EMD Millipore 1.25261.0001 Chromolith RP-18E Guard Cartridge is an indispensable tool for HPLC enthusiasts seeking to elevate their chromatographic endeavors. It effectively shields your analytical column from particulate contamination, extending its lifespan and ensuring consistent, reliable results. By safeguarding your samples, this guard cartridge empowers you to embark on your analytical journey with confidence.
Moreover, the EMD Millipore 1.25261.0001 Chromolith RP-18E Guard Cartridge optimizes chromatographic performance, resulting in sharper peaks and enhanced resolution. This remarkable enhancement in peak shape enables you to discern subtle details and unravel the intricacies of your samples with unparalleled precision. Invest in the EMD Millipore 1.25261.0001 Chromolith RP-18E Guard Cartridge today and unlock the gateway to impeccable HPLC performance.
PROS
- Superior peak shape and resolution for enhanced chromatographic separations.
- Exceptional inertness minimizes sample interactions, ensuring accurate and reliable results.
CONS
- May require specialized equipment for optimal performance.
- Cost may be higher compared to some alternative HPLC cartridges.
The EMD Millipore 1.50231.0001 Purospher Star RP-18 Endcapped LiChroCART 55-4 HPLC Cartridge is an exceptional choice for high-performance liquid chromatography (HPLC). Its advanced Purospher reversed-phase (RP-18) stationary phase provides unparalleled peak shape and resolution, enabling researchers to achieve optimal chromatographic separations. The endcapped nature of the stationary phase minimizes sample interactions, ensuring accurate and reliable results. This makes the Purospher cartridge ideal for a wide range of analytical applications, including the analysis of complex biological samples and pharmaceutical formulations.
The Purospher Star RP-18 Endcapped LiChroCART HPLC Cartridge is constructed with high-quality materials and designed to withstand the rigors of demanding HPLC environments. Its robust construction ensures consistent performance over extended periods of use. The cartridge is also fully compatible with standard HPLC systems, making it a versatile addition to any laboratory. Whether you're a seasoned chromatographer or just starting out, the EMD Millipore Purospher Star RP-18 Endcapped LiChroCART HPLC Cartridge is an excellent choice for achieving precise and reliable results.
PROS
- Delivers high-resolution separations of polar and hydrophilic compounds.
- Features a hydrophilic interaction chromatography (HILIC) stationary phase for enhanced selectivity.
CONS
- May require specialized solvents for certain applications.
- Can be more expensive than traditional reversed-phase columns.
The EMD Millipore SeQuant ZIC-HILIC HPLC Column is an exceptional choice for chromatographers seeking high-resolution separations of polar and hydrophilic compounds. Its unique hydrophilic interaction chromatography (HILIC) stationary phase provides enhanced selectivity for compounds that are challenging to retain using traditional reversed-phase chromatography. The column's 3.5µm particle size and 150 x 0.3 mm dimensions ensure efficient separations with minimal backpressure.
This column is particularly well-suited for the analysis of compounds such as carbohydrates, amino acids, peptides, and polar natural products. Its ability to retain these compounds allows for detailed characterization and quantification. Additionally, the column is compatible with a wide range of mobile phase compositions, including aqueous buffers and organic solvents. This versatility makes it a valuable tool for a variety of applications in pharmaceutical, biotechnology, and environmental analysis.
EMD Millipore HPLC columns offer a comprehensive range of options tailored to meet the specific requirements of various analytical and preparative applications. Whether you require exceptional column stability, high-throughput capabilities, or optimal resolution, EMD Millipore's advanced HPLC columns deliver unparalleled performance. The Purospher and Chromolith columns provide versatility for diverse separations, ensuring accurate and reliable results. Choose EMD Millipore HPLC columns to elevate your chromatographic endeavors and achieve卓越的separation outcomes.
Frequently Asked Questions
What are the key advantages of using EMD Millipore HPLC columns?
EMD Millipore HPLC columns provide a range of benefits, including exceptional column stability, high-throughput capabilities, and optimal resolution. These advantages make them ideal for various analytical and preparative applications, ensuring accurate and reliable results.
What types of HPLC columns does EMD Millipore offer?
EMD Millipore offers two main types of HPLC columns: Purospher and Chromolith. Purospher columns are known for their exceptional stability and versatility, while Chromolith columns provide high-throughput capabilities and excellent resolution.
What are the applications of EMD Millipore HPLC columns?
EMD Millipore HPLC columns are suitable for a wide range of applications, including protein purification, peptide analysis, and pharmaceutical analysis. They are also ideal for various industries, such as biotechnology, environmental testing, and food analysis.
How do EMD Millipore HPLC columns compare to other brands?
EMD Millipore HPLC columns are widely recognized for their high quality and reliability. They offer a combination of performance, versatility, and affordability, making them a preferred choice among researchers and scientists.
Where can I purchase EMD Millipore HPLC columns?
EMD Millipore HPLC columns are available from a variety of authorized distributors worldwide. You can find a list of distributors on the EMD Millipore website.
